منابع مشابه
New enzymatic assay of iron in serum.
A new enzymatic method for assaying iron in serum samples, suitable for automated analyzers, is reported. Three reagent mixtures are used: dilution buffer (pH 3.0; ascorbate), reagent 1 (pH 6.7; apoaconitase), and reagent 2 (pH 7.7; citrate, magnesium, and isocitrate dehydrogenase). Sera are diluted with dilution buffer. Fe3+ is liberated from transferrin in sera under acidic conditions, and th...
متن کاملEnzymatic assay of calcium in serum with phospholipase D.
(10) of 2.0 g/L and Holt et al. (12) of Ͻ3.0 g/L. HPLC-MS has no detectable interference with respect to endogenous compounds. The quantification limits based on acceptable imprecision, recovery, and ability to discriminate from potential interferences within the calibration range of the MEIA are 3.0 g/L (the lower limit of quantification) to 22.0 g/L. We have demonstrated that a higher lim...
متن کاملNew enzymatic assay using phospholipase D to measure total calcium in serum.
1. Pearson TC, Messinezy M, Westwood N, Green AR, Bench AJ, Huntly BJ, et al. Polycythemia vera updated: diagnosis, pathobiology, and treatment. Hematology (Am Soc Hematol Educ Program) 2000:51–68. 2. Tefferi A. Polycythemia vera: a comprehensive review and clinical recommendations. Mayo Clin Proc 2003;78:174–94. 3. Birgegard G, Wide L. Serum erythropoietin in the diagnosis of polycythaemia and...
متن کاملNew reagent for colorimetric assay of calcium in serum.
We report a new single-reagent colorimetric assay for the determination of calcium in serum. The assay is based on a novel chromogenic tetracarboxylic acid chelating agent. The method has several advantages over the o-cresolphthalein complexone (CPC) method, including improved linearity, better accuracy, increased stability, and lower working pH (9.0). The calibration curve for the assay is lin...
متن کاملNew enzymatic assay for glycohemoglobin.
BACKGROUND Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl amino acid and generates hydrogen peroxide, which reacts with chromogens in...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1996
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/42.8.1202